Sanger sequencing services

How to order →

• Purification of unpurified PCR products for Sanger sequencing
• Sanger sequencing (purified PCR products and plasmid samples)
  Read length
  • Max 1000 bp read, on average 800 bp
  • Recommended length for good quality data is above 120 bp
  Time schedule
  • Normal: 2 to 5 days, orders will be processed upon arrival and data sent when available in our LIMS.
  • Prior: orders will be processed immediately upon arrival and data will be sent when run is finished (orders arrived before 15:00 will be processed the same day)
  Delivered data
  • Raw (ABI files), analyzed (text files), quality scores
  • Data transfer via secured website (https) or attached to email
  
  
• Extra treatment for hairpin structures and high GC content
• UV/VIS template quantitation
• Fluorometric template quantitation
• Plasmid extraction and sequencing
• Genotyping services (only loading service)
• Use of primers provided by NSF (free of charge):
  
  

  Name	Sequence	Full Name
  M13FS	TGTAAAACGACGGCCAGT	M13 Forward
  M13RS	CAGGAAACAGCTATGACC	M13 Reverse
  SP6	TATTTAGGTGACACTATAG	SP6 primer
  T7	TAATACGACTCACTATAGGG	T7 primer
  T7term	TATGCTAGTTATTGCTCAG	T7 terminator
  T3	ATTAACCCTCACTAAAGGGA	T3 promotor

• Free storage of customer primers
• Up to 3 months template storage
• Minimum of 6 months sequencing data storage

If you require loading service (fragment analysis), please deliver the reactions to the NSF in clearly labeled 96 well plates.
For the reactions, please use primers with fluorescent dyes FAM, VIC, NED, PET and/or LIZ.